Job Title: Scientist I, Protein Sciences

Work Location: Middlesex County, Massachusetts

Summary:

Seeking a motivated and adaptable research scientist to join a Protein Sciences group. This laboratory-based position involves maintaining cell culture workflows, performing DNA plasmid preparations, expressing and purifying biologics, and conducting characterization assays to support a discovery pipeline. The ideal candidate is highly organized, process-oriented, and capable of analyzing, interpreting, and presenting experimental results to project teams.

Responsibilities:

• Execute expression and purification of biologic candidates, including monoclonal antibodies, from mammalian cell cultures.
• Participate in the design and development of purification methods for monoclonal antibodies, Fc-fusion proteins, and bispecific molecules.
• Conduct bacterial and mammalian cell culture for DNA preparation and protein expression.
• Perform routine cloning and plasmid preparation tasks to support laboratory workflows.
• Utilize FPLC systems to express and purify proteins at various scales, from milligram to gram quantities.
• Support quality analysis and characterization of biologics through established assays and protocols.
• Maintain laboratory equipment and ensure accurate, detailed electronic record-keeping of experiments.
• Collaborate with a diverse team of scientists, supporting multiple pipeline projects and adapting to new challenges as needed.

Qualifications:

• PhD or BA/BS/MS in chemistry, biochemistry, or related field with a minimum of 6+ years of relevant experience; pharmaceutical or biotech experience preferred.
• Proficiency in molecular biology techniques such as Gibson cloning, plasmid preparation, and sequence analysis.
• Experience with bacterial and mammalian cell culture, including transformation and transfection (HEK/CHO).
• Familiarity with generating and maintaining stable CHO cell lines is preferred.
• Expertise in protein purification techniques, including AKTA FPLC, affinity, IMAC, and preparative SEC.
• Knowledge of protein analysis methods such as endotoxin assays, analytical SEC, and SDS-PAGE/CE-SDS.
• Strong organizational skills for planning and executing experiments, with the ability to communicate results effectively.
• Excellent interpersonal, communication, and presentation skills, with the ability to work collaboratively in a team-oriented, matrix environment.

Published Category: Discovery Research & Preclinical